Device

Part:BBa_K176236:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-13)


For characterization of K176226: VR7+pCon 0.36->K176226


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
    Illegal NheI site found at 926
    Illegal NheI site found at 949
    Illegal NheI site found at 1775
    Illegal NheI site found at 1798
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3242
    Illegal BsaI.rc site found at 2753


Design Notes

VR7+pCon 0.36 as input for characterization of K176226.

VR7+pCon 0.36->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)


Source

K176102 K176226

References